Rabbit Anti-Human EGFR (pTyr869) Polyclonal: ATTO 594
A phospho-specific peptide corresponding to residues surrounding Tyr869 of human EGFR (AA866-873)
Human EGFR (pTyr869)
Antibody's full description:
Rabbit Anti-Human EGFR (pTyr869) Polyclonal
Antibody's other name:
Avian erythroblastic leukemia viral (v erb b) oncogene homolog Antibody, Cell growth inhibiting protein 40 Antibody, Cell proliferation inducing protein 61 Antibody, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) Antibodyy, Epidermal growth factor receptor Antibody, ERBB Antibody, ERBB1 Antibody, Errp Antibody, HER1Antibody, mENA Antibody, NISBD2 Antibodyy, PIG61 Antibody, SA7 Antibody, wa2 Antibody, Wa5 Antibody
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 134.277 kDa.
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-961 was sufficient for detection of EGFR (pTyr869) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cell membrane, Endoplasmic Reticulum, Golgi Apparatus, Endosome, Nucleus
See included datasheet or contact our support service.
EGFR is a receptor-tyrosine kinase that is activated upon binding epidermal growth factor (EGF) and transforming growth factor-alpha (TGFalpha). Binding of EGF ellicits a pro-growth and anti-apoptotic response through the Ras pathway. Missense mutations and deletions are common in a variety of cancers.
Refer to PubMed
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Country of production:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.