Catalog number:
SMC-416D-A390
Name:
Mouse Anti-Mouse Dicer Monoclonal IgG1, Clone S167-7
Size:
100 µg
Supplier:
StressMark antibodies
Price:
397.00
Clone:
S167-7
Immunogen:
Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
Antibody's full description:
Mouse Anti-Mouse Dicer Monoclonal IgG1 Antibody, Clone: S167-7: ATTO 390
Antibody's category:
Monoclonal Antibodies
Antibody's other name:
Dicer1 Antibody, DCR Antibody, DCR1 Antibody, DCR-1 Antibody, Double-strand-specific ribonuclease Antibody, Helicase with RNase motif Antibody, HERNA Antibody, Helicase MOI Antibody, KIAA0928 Antibody, Endoribonuclease Dicer Antibody
Raised in:
Mouse
Antibody's target:
Dicer
Primary research fields:
Cell Signaling, Epigenetics
Brandname:
None
Antibodies' applications:
WB, ICC/IF
Antibody's reactivity:
Human, Mouse, Rat
Antibody's dilutions:
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purity:
Protein G Purified
Antibody buffer for storage:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Antibody's concentration:
1 mg/ml
Antibody's specificity:
Detects ~215kDa.
Storage recommendations:
-20ºC
Shipping recommendations:
Blue Ice or 4ºC
Antibody certificate of analysis:
1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm
Tissue specificity:
See included datasheet or contact our support service
Scientific context:
Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)
Bibliography:
1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60. 2. Lee Y., et al. (2003) Nature 425: 415-9. 3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.
Released date:
5-Feb-2013
Tested applications:
To be tested
Tested reactivity:
To be tested
NCBI number:
NP_683750.2
Gene number:
192119
Protein number:
Q8R418
Antibody's datasheet:
Contact our support service
Representative figure link:
|
Representative figure legend:
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (SMC-416). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (SMC-416) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Dicer Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (SMC-416). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (SMC-416) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa. Mouse Anti-Dicer Antibody [S167-7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-416) Mouse Anti-Dicer Antibody [S167-7] used in Western Blot (WB) on Rat Brain Membrane (SMC-416)
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availability:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test:
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:
Mus musculus