Catalog number:
SPC-1076D-RPE
Name:
Rabbit Anti-Human RSK1 (pSer221) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Ser221 of human RSK1 (AA218-224)
Antibody's target:
Human RSK1 (pSer221)
Antibody's full description:
Rabbit Anti-Human RSK1 (pSer221) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
RPS6KA1 Antibody, RSK 1 Antibody, RSK 1 p90 Antibody, RSK Antibody, RSK-1 Antibody, S6K alpha 1 Antibody, S6K-alpha-1 Antibody, 90 kDa ribosomal protein S6 kinase 1 Antibody, Ribosomal protein S6 kinase 90kD 1 Antibody, Ribosomal protein S6 kinase 90kDa polypeptide 1 Antibody, Ribosomal protein S6 kinase alpha 1 Antibody, Ribosomal protein S6 kinase alpha-1 Antibody, Ribosomal protein S6 kinase polypeptide 1 Antibody, Ribosomal S6 kinase 1 Antibody, RPS6K1 alpha Antibody, rps6ka Antibody, EC 2.7.11.1 Antibody, HU 1 Antibody, HU1 Antibody, KS6A1_HUMAN Antibody, MAP kinase activated protein kinase 1a Antibody, MAP kinase-activated protein kinase 1a Antibody, MAPK-activated protein kinase 1a Antibody, MAPKAP kinase 1a Antibody, MAPKAPK-1a Antibody, MAPKAPK1A Antibody, MGC79981 Antibody, Mitogen-activated protein kinase-activated protein kinase 1A Antibody, OTTHUMP00000004113 Antibody, p90 RSK1 Antibody, p90-RSK 1 Antibody, p90rsk Antibody, p90RSK1 Antibody, p90S6K Antibody, pp90RSK1 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 82.723 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-1076 was sufficient for detection of RSK1 (pSer221) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Nucleus, Cytoplasm
Tissue specificity:
Widely expressed with higher expression in lympocytes, granulocytes, leukocytes, macrophages, blood, and cerebrospinal fluid cells.
Scientific context:
RSK1 is a RSK (ribosomal S6 kinase) family of serine/threonine kinases. RSK1 contains 2 nonidentical kinase catalytic domains. RSK1 is known phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway -- a pro-growth/anti-apoptotic pathway, as it will activate CREB, which in turn will upregulate expression of cylin-D and c-Myc. Activation of this pathway is common in various types of tumours. In some cases, such breast and prostate cancer, upreguation of RSK has been shown in some patients.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_002944
Gene number:
6195
Protein number:
Q15418
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus