Catalog number:
SPC-1071D-RPE
Name:
Rabbit Anti-Human RAF1 (pSer296) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Ser296 of human RAF1 (AA293-299)
Antibody's target:
Human RAF1 (pSer296)
Antibody's full description:
Rabbit Anti-Human RAF1 (pSer296) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
c Raf Antibody, C-raf Antibody, C-Raf proto-oncogene, serine/threonine kinase Antibody, CMD1NN Antibody, Craf 1 transforming gene Antibody, cRaf Antibody, Craf1 transforming gene Antibody, EC 2.7.11.1 Antibody, kinase Raf1 Antibody, Murine sarcoma 3611 oncogene 1 Antibody, NS5 Antibody, Oncogene MIL Antibody, Oncogene RAF1 Antibody, OTTHUMP00000160218 Antibody, OTTHUMP00000207813 Antibody, OTTHUMP00000209389 Antibody, Protein kinase raf 1 Antibody, Proto-oncogene c-RAF Antibody, Raf 1 Antibody, Raf 1 proto oncogene serine/threonine kinase Antibody, RAF Antibody, Raf proto oncogene serine/threonine protein kinase Antibody, RAF proto-oncogene serine/threonine-protein kinase Antibody, RAF-1 Antibody, RAF1 Antibody, RAF1_HUMAN Antibody, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 Antibody, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV Antibody, v raf 1 murine leukemia viral oncogene homolog 1 Antibody, v-raf murine sarcoma viral oncogene homolog 1 Antibody, vraf1 murine leukemia viral oncogene homolog 1 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects ~73kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-1071 was sufficient for detection of RAF1 (pSer296) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm, Cell Membrane, Mitochondrion, Nucleus
Tissue specificity:
In skeletal muscle, isoform 1 is more abundant than isoform 2
Scientific context:
RAF1 is a protein-serine/threonine kinase. RAF-1 is upregulated in many cancers since Ras (an upstream activator of RAF) is mutated/upreguated in many cancers. For example, RAF-1 is over-expressed due to oncogenic ras mutations in about 35% of Non-Small Cell Lung Cancer (NSCLC). A small number of RAF mutations have been found in stomach and mouth tumours. RAF-1 gene amplification was detected in 4% of bladder cancer samples, while deletions at the RAF-1 locus were detected in 2.2% of these samples; these amplifications and deletions were correlated with tumour grade.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_002871
Gene number:
5894
Protein number:
P04049
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus