Catalog number:
SPC-1050D-RPE
Name:
Rabbit Anti-Human PDK1 (pSer241) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Ser241 of human PDK1 (AA238-244)
Antibody's target:
Human PDK1 (pSer241)
Antibody's full description:
Rabbit Anti-Human PDK1 (pSer241) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
PDK1 Antibody, PDPK1 Antibody, PDPK1_HUMAN Antibody, PDPK2 Antibody, PkB kinase Antibody, PkB kinase like gene 1 Antibody, PkB like 1 Antibody, PRO0461 Antibody, Protein kinase Antibody3 phosphoinositide dependent protein kinase 1 Antibody, 3-phosphoinositide-dependent protein kinase 1 Antibody, hPDK 1 Antibody, hPDK1 Antibody, MGC20087 Antibody, MGC35290 Antibody, OTTHUMP00000159109 Antibody, OTTHUMP00000159110 Antibody, OTTHUMP00000174525 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects ~63kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-1050 was sufficient for detection of PDK1 (pSer241) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm, Nucleus, Cell Membrane, Cell Junction, Focal Adhesion
Tissue specificity:
Expressed ubiquitously with highest expression in blood vessels and the brain. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.
Scientific context:
See included datasheet or contact our support service.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_002601
Gene number:
5170
Protein number:
O15530
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus