Rabbit Anti-Human PAK1 (pSer144) Polyclonal: RPE
A phospho-specific peptide corresponding to residues surrounding Ser144 of human PAK1 (AA141-147)
Human PAK1 (pSer144)
Antibody's full description:
Rabbit Anti-Human PAK1 (pSer144) Polyclonal
Antibody's other name:
ADRB2 Antibody, Alpha PAK Antibody, Alpha-PAK Antibody, p21 activated kinase 1 Antibody, p21 protein (Cdc42/Rac) activated kinase 1 Antibody, p21-activated kinase 1 Antibody, p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) Antibody, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) Antibody, p65 PAK Antibody, p65-PAK Antibody, P68-PAK Antibody, PAK alpha Antibody, PAK-1 Antibody, Pak1 Antibody, PAK1_HUMAN Antibody, Paka Antibody, PAKalpha Antibody, Protein kinase MUK2 Antibody, Rac/p21-activated kinase Antibody, Serine/threonine-protein kinase PAK 1 Antibody, STE20 homolog yeast Antibody
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 60.661 kDa.
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-1043 was sufficient for detection of PAK1 (pSer144) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm, Cell Junction, Focal Adhesion, Cell Membrane, Cell Projection, Invadopodium
Highly expressed in the brain and blood cells. Overexpressed in gastric cancer cells and tissues.
PAK1 is a protein-serine/threonine kinase. Pak1 binds to activated forms of the GTPases Cdc42 and Rac. Upon binding to Cdc42 or Rac, Pak1 is activated and autophosphorylates. Pak1 has dozens of substrates, activating cytoskeletal and transcription pathways that enhance cell motility, proliferation, and survival.
Refer to PubMed
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Country of production:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.