Catalog number:
SPC-1027D-RPE
Name:
Rabbit Anti-Human MEK3 (pTyr230) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Tyr230 of human MKK3 (AA228-233)
Antibody's target:
Human MEK3 (pTyr230)
Antibody's full description:
Rabbit Anti-Human MEK3 (pTyr230) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
Dual specificity mitogen-activated protein kinase kinase 3 antibody, MAP kinase kinase 3 antibody, map2k3 antibody, MAPK ERK kinase 3 antibody, MAPK/ERK kinase 3 antibody, MAPKK3 antibody, MEK 3 antibody, MEK3 antibody, Mitogen activated protein kinase kinase 3 antibody, MKK 3 antibody, MKK3 antibody, mMKK3b antibody, MP2K3_HUMAN antibody, PRKMK 3 antibody, SAPK kinase 2 antibody, SAPKK 2 antibody, Stress activated protein kinase kinase 2 antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 36.172 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-1027 was sufficient for detection of MEK3 (pTyr230) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm, Cytosol, Membrane, Nucleoplasm
Tissue specificity:
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Scientific context:
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_659731
Gene number:
5606
Protein number:
P46734
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus