Catalog number:
SPC-998D-RPE
Name:
Rabbit Anti-Human IKKa (pThr179+pSer180) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Thr179 and Ser180 of human IKKa (AA177-184)
Antibody's target:
Human IKKa (pThr179+pSer180)
Antibody's full description:
Rabbit Anti-Human IKKa (pThr179+pSer180) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
Chuk Antibody, CHUK1 Antibody, Conserved Helix Loop Helix Ubiquitous Kinase Antibody, I Kappa B Kinase 1 Antibody, IkB kinase alpha subunit Antibody, IkBKA Antibody, IKK 1 Antibody, IKK A Antibody, IKKA Antibody, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells Antibody, NFKBIKA Antibody, Nuclear Factor Kappa B Inhibitor Kinase Alpha Antibody, Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor Antibody, TCF16 Antibody, Transcription factor 16 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 84.654 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-998 was sufficient for detection of IKKa (pThr179+pSer180) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm, Nucleus
Tissue specificity:
Widely expressed.
Scientific context:
IKKa is a protein-serine/threonine kinase. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Loss of function therefore blunts the NF-kB response. Cells with DNA damage are unable to undergo apoptosis. Oncogenesis may therefore occur indirectly by inhibitting a downstream tumour suppresor (inhibitors of NF-kB).
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_001269
Gene number:
1147
Protein number:
O15111
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus