Catalog number:
SPC-991D-RPE
Name:
Rabbit Anti-Human TFIIF (pSer385 + pThr389) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Ser385 and Thr389 of human TFIIF (AA382-392)
Antibody's target:
Human TFIIF (pSer385 + pThr389)
Antibody's full description:
Rabbit Anti-Human TFIIF (pSer385 + pThr389) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
GTF2F1 Antibody, Transcription initiation factor IIF subunit alpha Antibody, 2810405L04Rik Antibody, BTF4 Antibody, C76800 Antibody, General transcription factor IIF 74 kDa subunit Antibody, MGC94148 Antibody, OTTHUMP00000237859 Antibody, RAP74 Antibody, T2FA_HUMAN Antibody, TF2F1 Antibody, GTF2F1 Antibody, TFIIF-alpha Antibody, Transcription initiation factor RAP74 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 58.24 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-991 was sufficient for detection of TFIIF (pSer385 + pThr389) in 10 µg of Jurkat cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Nucleus
Tissue specificity:
See included datasheet or contact our support service.
Scientific context:
TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_002087
Gene number:
2962
Protein number:
P35269
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus