Catalog number:
SPC-978D-RPE
Name:
Rabbit Anti-Human FAK (pTyr577) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Tyr577 of human FAK (AA574-580)
Antibody's target:
Human FAK (pTyr577)
Antibody's full description:
Rabbit Anti-Human FAK (pTyr577) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
FADK1 Antibody, FADK Antibody, FAK related non kinase polypeptide Antibody, FAK1 Antibody, FAK Antibody, Focal adhesion kinase 1 Antibody, Focal adhesion kinase isoform FAK Del33 Antibody, Focal adhesion kinase related nonkinase Antibody, FRNK Antibody, p125FAK Antibody, PPP1R71 Antibody, Protein phosphatase 1 regulatory subunit 71 Antibody, Protein tyrosine kinase 2 Antibody, Ptk2 Antibody, PTK2 protein tyrosine kinase 2 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 119.233 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-978 was sufficient for detection of FAK (pTyr577) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cell Junctions, Focal Adhesion, Cell Membrane
Tissue specificity:
Highly expressed in brain but found in heart, placenta, lung, kidney and skeletal muscle.
Scientific context:
FAK, also known as PTK2, is a non-receptor protein-tyrosine kinase that is activated by tyrosine-phosphorylation in response to integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands. It serves as adaptor protein. It is linked to the MAPK pathway via Grb2, and is also linked to PI3K and PLC gamma pathways. It plays a role as a scaffold for MDM2-mediated p53 degradation, and has been implicated in regulation of cell-cell contacts, microtubule stability and control of gene transcription. FAK has high expression levels in various human cancers as it promotes tumour cell motility, matrix degradation, angiogenesis and suppresses apoptosis, anoikis and senescence.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_722560
Gene number:
5747
Protein number:
Q05397
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus