Catalog number:
SPC-964D-RPE
Name:
Rabbit Anti-Human EML4 (pTyr226) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Tyr226 of human EML4 (AA223-229)
Antibody's target:
Human EML4 (pTyr226)
Antibody's full description:
Rabbit Anti-Human EML4 (pTyr226) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
C2orf2 Antibody, Echinoderm microtubule associated protein like 4 Antibody, ELP 120 Antibody, EMAL4_HUMAN Antibody, EMAP4 Antibody, Restrictedly overexpressed proliferation associated protein Antibody, ROPP120 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 108.903 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-964 was sufficient for detection of EML4 (pTyr226) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoskeleton
Tissue specificity:
See included datasheet or contact our support service.
Scientific context:
Echinoderm microtubule-associated (EML) proteins function to modify the assembly dynamics of microtubules. EML4 (Echinoderm microtubule-associated protein-like 4), also known as EMAPL4, ELP120, C2orf2 or ROPP120, is a 981 amino acid cytoplasmic protein that contains nine WD repeats. Expressed at high levels during mitosis, EML4 is thought to modify the assembly dynamics of microtubules, specifically altering microtubules to become longer and more flexible. Due to a chromosomal inversion with chromosome 2p, EML4 may exist as a fusion protein with ALK (anaplastic lymphoma receptor tyrosine kinase), producing an EML4-ALK fusion complex that plays a role in the pathogenesis of lung cancer.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_001138548
Gene number:
27436
Protein number:
Q9HC35
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus