Catalog number:
SPC-963D-RPE
Name:
Rabbit Anti-Human PERK (pThr892) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Thr892 of human PERK (AA889-895)
Antibody's target:
Human PERK (pThr892)
Antibody's full description:
Rabbit Anti-Human PERK (pThr892) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
Eif2ak3 Antibody, Eukaryotic translation initiation factor 2 alpha kinase 3 Antibody, Heme regulated EIF2 alpha kinase Antibody, HRI Antibody, HsPEK Antibody, Pancreatic eIF2-alpha kinase Antibody, PEK Antibody, PRKR-like Endoplasmic Reticulum kinase Antibody, WRS Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 125.147 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-963 was sufficient for detection of PERK (pThr892) in 10 µg of HepG2 cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Endoplasmic Reticulum Membrane
Tissue specificity:
See included datasheet or contact our support service.
Scientific context:
PERK (eukaryotic translation initiation factor 2-α kinase 3, eIF2α kinase 3, PEK, WRS) is a Ser/Thr protein kinase, type I membrane protein of the endoplasmic reticulum that is abundant in secretory tissues and is inducible by ER-stress. PERK phosphorylates the α subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, a reduction of translation initiation and repression of global protein synthesis. PERK may serve as an effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. PERK dimerizes with HSPA5/BIP in resting cells and oligomerizes in ER-stressed cells. Perturbation in protein folding in the endoplasmic reticulum (ER) can promote dissociation from HSPA5/BIP and oligomerization, resulting in autophosphorylation and kinase activity induction.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_004827
Gene number:
9451
Protein number:
Q9NZJ5
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus