Catalog number:
SPC-941D-RPE
Name:
Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Ser383 and Ser385 of human CrkRS (AA380-388)
Antibody's target:
Human CrkRS (pSer383 + pSer385)
Antibody's full description:
Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
arginine/serine-rich Antibody, Cdc2 related kinase arginine/serine rich Antibody, CDC2 related protein kinase 7 Antibody, Cdk12 Antibody, Cell division cycle 2 related protein kinase 7 Antibody, Cell division protein kinase 12 Antibody, CRK7 Antibody, Pksc Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 164.155 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-941 was sufficient for detection of CrkRS (pSer383 + pSer385) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Nucleus
Tissue specificity:
See included datasheet or contact our support service.
Scientific context:
CrkRS (Cdc2-related kinase, arginine/serine-rich, also designated CRK7 and CRKR) is an ubiquitous protein that appears to localize to the nucleus and link transcription and splicing machinery. CrkRS belongs to the serine/threonine protein kinase family and Cdc2/Cdkx subfamily. CrkRS has extensive proline-rich regions that resemble SH3 and WW domain binding sites, and an RS domain that is characteristic of splicing factors. The protein kinase domain of CrkRS is 89% identical to the CHED protein kinase, also designated CDC2L5 and cell division cycle 2-like 5 (cholinesterase-related cell division controller), however outside the kinase domains the two proteins are unique. Cell cycle control kinases can phosphorylate proteins important for differentiation and apoptosis and provide connections between proliferation, differentiation, apoptosis, and neurocytoskeleton dynamics.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_057591
Gene number:
51755
Protein number:
Q9NYV4
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus