Catalog number:
SPC-926D-RPE
Name:
Rabbit Anti-Human BRK (pSer446 + pTyr447) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Ser446 and Tyr447 of human BRK (AA443-450)
Antibody's target:
Human BRK (pSer446 + pTyr447)
Antibody's full description:
Rabbit Anti-Human BRK (pSer446 + pTyr447) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
Breast tumor kinase Antibody, Protein tyrosine kinase 6 Antibody, Ptk6 Antibody, PTK6_HUMAN Antibody, Tyrosine protein kinase BRK Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 51.834 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-926 was sufficient for detection of BRK (pSer446 + pTyr447) in 10 µg of human A549 cell line lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm, Nucleus
Tissue specificity:
See included datasheet or contact our support service.
Scientific context:
BRK otherwise known as PTK6, is a protein-tyrosine kinase. It is a cytoplasmic nonreceptor protein-kinase that seems to function as an intracellular signal transducer in epithelial tissues. Upregulation of this gene in mammary epithelium causes sensitization to epidermal growth factor (EGF) and results in a partially transformed phenotype. Upregulation of this gene has been found in some breast cancers, while mutation in this gene have been found in skin, ovairan, and gastric tumours.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_005966
Gene number:
5753
Protein number:
Q13882
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus