Catalog number:
SPC-919D-RPE
Name:
Rabbit Anti-Human Aurora A (pThr287 + pThr288) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Thr287 and Thr288 of human Aurora A (AA285-291)
Antibody's target:
Human Aurora A (pThr287 + pThr288)
Antibody's full description:
Rabbit Anti-Human Aurora A (pThr287 + pThr288) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
Aurora 2 Antibody, AIK Antibody, ARK1 Antibody, BTAK Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects ~48kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-919 was sufficient for detection of Aurora A (pThr287 + pThr288) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm, Cytoskeleton
Tissue specificity:
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Scientific context:
Aurora A is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_003591
Gene number:
6790
Protein number:
O14965
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus