Catalog number:
SPC-917D-RPE
Name:
Rabbit Anti-Human ASK1 (pSer1033) Polyclonal: RPE
Size:
100 µl
Supplier:
StressMark antibodies
Price:
394.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Ser1033 of human ASK1 (AA1030-1036)
Antibody's target:
Human ASK1 (pSer1033)
Antibody's full description:
Rabbit Anti-Human ASK1 (pSer1033) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
Apoptosis signal regulating kinase 1 Antibody, ASK-1 Antibody, M3K5 Antibody, MAPK/ERK Kinase Antibody, MEKK5 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 154.537 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-917 was sufficient for detection of ASK1 (pSer1033) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm, Endoplasmic reticulum
Tissue specificity:
Abundantly expressed in heart and pancreas.
Scientific context:
ASK1 is part of the MK Kinases, also called MAP kinase kinase kinases, and they phosphorylate and activate the MAP kinases, including ERK, JNK and p38. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_005914
Gene number:
4217
Protein number:
Q99683
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus