Rabbit Anti-Human Met (pTyr1230) Polyclonal: APC
A phospho-specific peptide corresponding to residues surrounding Tyr1230 of human Met (AA1229-1235)
Human Met (pTyr1230)
Antibody's full description:
Rabbit Anti-Human Met (pTyr1230) Polyclonal
Antibody's other name:
AUTS9 antibody, c met antibody, D249 antibody, Hepatocyte growth factor receptor antibody, HGF antibody, HGFR antibody, MET antibody, Met proto oncogene tyrosine kinase antibody, MET_HUMAN antibody, Oncogene MET antibody, Par4 antibody, Proto-oncogene c-Met antibody, RCCP2 antibody, Scatter factor receptor antibody, SF receptor antibody, Tyrosine-protein kinase Met antibody
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 155.527 kDa.
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-1024 was sufficient for detection of Met (pTyr1230) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain.
MET is a receptor-tyrosine kinase that is activated upon binding hepatocyte growth factor (HGF). MET/HGF signalling has been implicated in the generation and progression of a variety of tumours. The coexpression of wild type MET and HGF in the same cell (which generates an autocrine stimulatory loop) induces oncogenic transformation. All investigated naturally occurring MET mutants show increased tyrosine phosphorylation level and enhanced kinase activity
Refer to PubMed
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Country of production:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.