Rabbit Anti-Human MAPKAPK2 (pThr222) Polyclonal: APC
A phospho-specific peptide corresponding to residues surrounding Thr222 of human MAPKAPK2 (AA219-226)
Human MAPKAPK2 (pThr222)
Antibody's full description:
Rabbit Anti-Human MAPKAPK2 (pThr222) Polyclonal
Antibody's other name:
MAP kinase activated protein Kinase 2 antibody, MAPK2_HUMAN antibody, MAPKAP K2 antibody, MAPKAP kinase 2 antibody, MAPKAPK2 antibody, Mitogen activated protein kinase activated protein kinase 2 antibody, MK 2 antibody, MK2 antibody
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 45.568 kDa.
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-1014 was sufficient for detection of MAPKAPK2 (pThr222) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Expressed in all tissues examined.
MAPKAPK2 is a protein-serine/threonine kinase regulated through direct phosphorylation by p38 MAPK. It is involved in a number of cellular processes such as inflammation, stress responses, nuclear export, cell proliferation, and regulation of gene expression. It mediates both ERK and p38 MAPK-dependent neutrophil responses, participates in TNFα-stimulated exocytosis of secretory vesicles in neutrophils, and plays a role in phagocytosis-induced respiratory burst activity. Another one of its major substrates is HSP27, a protein responsble for stimulating actin polymerization in order to facilitate the recovery from cytoskeleton destruction during cellular stress. Over-expression of the gene has been linked to Multiple Myeloma resistance to chemotherapy, and it has been found to play a role in neuroinflammatory and neurodegenerative diseases such as Alzheimer's.
Refer to PubMed
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Country of production:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.