Catalog number:
SPC-977D-APC
Name:
Rabbit Anti-Human ERK5 (pThr219 + pTyr221) Polyclonal: APC
Size:
100 µl
Supplier:
StressMark antibodies
Price:
396.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Thr219 and Tyr221 of human ERK2 (AA 216-222)
Antibody's target:
Human ERK5 (pThr219 + pTyr221)
Antibody's full description:
Rabbit Anti-Human ERK5 (pThr219 + pTyr221) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
Big MAP kinase 1 Antibody, BMK 1 kinase Antibody, ERK5 Antibody, BMK1 Antibody, EC 2.7.11.24 Antibody, ERK4 Antibody, ERK5 Antibody, Extracellular signal regulated kinase 5 Antibody, MAP kinase 7 Antibody, MAPK7 Antibody , Mitogen activated protein kinase 7 Antibody, MK07_HUMAN Antibody, OTTHUMP00000065906 Antibody, OTTHUMP00000065907 Antibody, PRKM7 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 88.637 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-977 was sufficient for detection of ERK5 (pThr219 + pTyr221) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Cytoplasm, Nucleus
Tissue specificity:
Highly expressed in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver
Scientific context:
ERK5 or MAPK7 is a protein-serine/threonine kinase. ERK5 is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). Upregulation of this gene has been found in breast, prostate, and liver cancer.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_002740.2
Gene number:
5598
Protein number:
Q13164
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus