Catalog number:
SPC-950D-APC
Name:
Rabbit Anti-Human Chk2 (pThr383) Polyclonal: APC
Size:
100 µl
Supplier:
StressMark antibodies
Price:
396.00
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Thr383 of human Chk2 (AA380-386)
Antibody's target:
Human Chk2 (pThr383)
Antibody's full description:
Rabbit Anti-Human Chk2 (pThr383) Polyclonal
Antibody's category:
Polyclonal Antibodies
Antibody's other name:
Cds1 homolog Antibody, Checkpoint kinase 2 Antibody, CHEK 2 Antibody, CHK2 checkpoint homolog Antibody, CHK2_HUMAN Antibody, hCds1 Antibody, LFS 2 Antibody, PP1425 Antibody, RAD 53 Antibody, Serine/threonine protein kinase Chk2 Antibody
Verified applications:
WB, AM
Raised in:
Rabbit
Antibody's reactivity:
Human
Antibody's recommended dilutions for use:
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from:
Peptide Affinity Purified
Recommended buffer for storage:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration:
See included datasheet or contact our support service
Antibody's specificity:
Detects 60.915 kDa.
Storage recommendations:
-20°C
Shipping recommendations:
Blue Ice or 4°C
Certificate of analysis:
A 1:250 dilution of SPC-950 was sufficient for detection of Chk2 (pThr383) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:
Nucleus
Tissue specificity:
See included datasheet or contact our support service.
Scientific context:
Chk2 is a protein kinase involved in cell cycle arrest in response to DNA damage. CHK2 is activated through phosphorylation by ATM and ATR at T68. Activated CHK2 phosphorylates and inhibits CDC25 a dual-specificity phosphatase required for progression through the cell cycle. CHK2 further causes cell cycle earrest through activation of a DNA repair protein BRCA1 and apoptosis factor PML. The role of CHK2 in the development of cancer is in a primarily tumour suppressor role through causing cell cycle arrest. It has been observed that inhibition of CHK2 through mutations in the regulatory FHA domain has been observed in low frequency in osteosarcomas and cancers of the ovary, lung and vulva. Another mutation, "1100delC" has been commonly found in breast cancers.
Bibliography:
Refer to PubMed
Released date:
29/Aug/2016
NCBI number:
NP_009125
Gene number:
11200
Protein number:
O96017
PubMed number:
Refer to PubMed
Tested applications:
To be tested
Tested reactivity:
To be tested
Antibody's datasheet:
Contact our support service to receive datasheet or other technical documentation.
Representative figure link:
No Data Available
Warning information:
Non-hazardous
Country of production:
Canada
Total weight (kg):
1.4
Net weight (g):
0.1
Stock availabilit:
In Stock
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:
Polyclonals and antibodies
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:
Oryctolagus cuniculus