Catalog number:
E21-357
Name:
HMGB1/HMG-1
Size:
10ug
Supplier:
EnoGene
Price:
375.00
Shipping_Temperature:
Ambient
Product_State:
Lyophilized
Formulation_Description:
Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.
Storage:
Lyophilized protein should be stored at <-20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at <-20°C for 3 months.
Reconstitution:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Background:
High mobility group protein B1 is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile, it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte, T cell, dendritic cell activities in inflammatory responses.
Alternative Names:
High Mobility Group Protein B1; High Mobility Group Protein 1; HMG-1; HMGB1; HMG1
Expression system:
Human cells