Catalog number:
E21-029
Name:
EGF
Size:
10ug
Supplier:
EnoGene
Price:
375.00
Shipping_Temperature:
Ambient
Product_State:
Lyophilized
Formulation_Description:
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, pH 7.0.
Storage:
Lyophilized protein should be stored at <-20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at <-20°C for 3 months.
Reconstitution:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Background:
Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.
Alternative Names:
Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
Expression system:
E.coli